We previously reported on the secretion of Streptomyces mobaraensis transglutaminase by Corynebacterium glutamicum ATCC13869 (formerly classified as Brevibacterium lactofermentum). In the present work, we investigated whether any other coryneform bacteria showed higher productivity than C. glutamicum ATCC13869. We found that most coryneform species secreted pro-transglutaminase efficiently. Moreover, we confirmed that Corynebacterium ammoniagenes ATCC6872 produced about 2.5 g/l pro-transglutaminase over a 71-h period in a jar fermentor. Our findings suggest that some other coryneform bacteria, especially C. ammoniagenes ATCC6872, are potential hosts for industrial scale protein production.
展开▼
机译：我们之前曾报道过谷氨酸棒杆菌ATCC13869（以前被称为乳铁杆短杆菌）分泌的莫巴链霉菌转谷氨酰胺酶的分泌。在目前的工作中，我们调查了是否有其他棒状细菌显示出比谷氨酸棒杆菌ATCC13869更高的生产率。我们发现大多数棒状物种有效地分泌前转谷氨酰胺酶。此外，我们证实在71个小时的发酵罐中，产氨棒杆菌ATCC6872产生了约2.5 g / l的转谷氨酰胺原酶。我们的发现表明，其他一些棒状细菌，尤其是产氨梭菌ATCC6872，是工业规模蛋白质生产的潜在宿主。
展开▼
